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If a Histotechnician received a fresh sample and the pathologist's order is to have a study on the mitochondria. What choice of stain can be best used for staining the mitochondria?


A) Neutral red
B) Janus green
C) Nile blue
D) Thionine

E) B) and D)
F) A) and D)

Correct Answer

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Which of the following choices is a metachromatic dye?


A) Iodine
B) Malachite green
C) Methyl green
D) Methylene violet

E) A) and D)
F) B) and C)

Correct Answer

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This stain is useful in the detection of myelofibrosis?


A) Methyl-Green Pyronin
B) Perl's stain
C) Reticulin stain
D) Toluidine

E) B) and C)
F) A) and C)

Correct Answer

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What is the possible reason why Orcein is not used as a cytological stain?


A) It is a synthetic dye.
B) It is a natural dye.
C) It has a poor staining property.
D) It is a colorless substance.

E) C) and D)
F) A) and D)

Correct Answer

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All of the following are standard MSB techniques for fibrin except for:


A) Martius Yellow
B) Brilliant crystal scarlet
C) Soluble blue
D) Verhoeff's

E) A) and B)
F) A) and C)

Correct Answer

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What dye is used to stain hemoglobin?


A) Aniline blue
B) Carbol-fuschin
C) Benzidine
D) Congo red

E) All of the above
F) A) and C)

Correct Answer

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Melanin is best demonstrated by what staining technique?


A) Von Kossa's silver nitrate method
B) Masson Fontana silver technique
C) Lindquist's Modified Rhodanine technique
D) None of the above

E) B) and C)
F) A) and D)

Correct Answer

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In Hematoxylin staining solutions, what are the mordants used to demonstrate nuclear and cytoplasmic structures?


A) Alum
B) Copper
C) Iron
D) Both A and C

E) B) and D)
F) None of the above

Correct Answer

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In the combined Alcian Blue-PAS Technique for acid and neutral mucins, this alum hematoxylin solution should not be use as a counterstain. What is this alum hematoxylin solution?


A) Ehrlich's hematoxylin
B) Harris hematoxylin
C) Cole's hematoxylin
D) Mayer's hematoxylin

E) B) and C)
F) A) and D)

Correct Answer

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After halfway done with staining, the histotechnician accidentally broke the slide. And this slide is needed by the Pathologist as soon as possible for immediate examination. What must a histotechnician do with the broken slide?


A) Mount the slide to another clean xylene-moist slide with a drop of Clarite.
B) Cut a new section, mount and stain.
C) Inform the Pathologist that the slide is broken and you need to prepare a new one.
D) Placed together the broken slide.

E) None of the above
F) A) and B)

Correct Answer

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This is not essential to the chemical union of the tissue and the dye and it does not participate in the staining reaction. What is this substance?


A) Mordant
B) Accentuator
C) Water
D) Aniline water

E) A) and C)
F) B) and C)

Correct Answer

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The staining done by Janus Green B to demonstrate mitochondria is an example of what type of staining method?


A) Vital staining
B) Counterstaining
C) Direct staining
D) None of the above

E) B) and D)
F) B) and C)

Correct Answer

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It is an oxazine dye used as an alternative to iron hematoxylin nuclear stain?


A) Celestine blue
B) Trypan blue
C) Nile blue
D) Toluidine blue

E) A) and B)
F) All of the above

Correct Answer

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Trypan blue vitally stains which specific cell structure?


A) Cytoplasm
B) Mitichondria
C) Nucleus
D) Reticulo-Endothelial System

E) B) and D)
F) All of the above

Correct Answer

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This natural dye is an excellent stain for elastic fibers and is especially recommended in dermatological studies?


A) Toluidine blue
B) Neutral red
C) Carmine
D) Orcein

E) A) and B)
F) C) and D)

Correct Answer

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What group of dyes is insoluble in water but soluble in alcohol?


A) Acid dyes
B) Basic dyes
C) Neutral dyes
D) None of the above

E) A) and B)
F) A) and C)

Correct Answer

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This is a natural dye derived through the extraction from the heartwood of a Mexican tree.


A) Orcein
B) Saffron
C) Hematoxylin
D) Cochineal dye

E) B) and D)
F) A) and C)

Correct Answer

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This stain is best known as an indicator but can be used to stain axis cylinders in embryos?


A) Congo red
B) Crystal violet
C) Celestine blue
D) Neutral red

E) C) and D)
F) B) and D)

Correct Answer

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This dye is an essential component of the Schiff reagent used in histochemical staining of carbohydrates?


A) Acid fuchsin
B) Basic fuchsin
C) Carmine
D) Aniline blue

E) A) and C)
F) All of the above

Correct Answer

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What part of a living cell is considered to be resistant to vital stains?


A) Nucleus
B) Mitochondria
C) RES
D) None of the above

E) B) and D)
F) A) and D)

Correct Answer

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